um I'm Judy Cannon I'm the core facility director and I just want to start with abuse like just discussing the um Center um and then I'll be followed by the technical director who will get more into the details of the actual Court facility and so um I wanted to introduce our Center of top 10 information metabolism Center which is the center of biomedical research Excellence funded by the NIH and our Center is focused on the three um letters of aim I'll talk to the information metabolism our focus is on basic research leading into translational studies and that um one of the major roles of the goals of the center is to support Junior faculty um giving them mentoring as well as the necessary equipment and Technical expertise through the core facility to get them to um r01 external level funding but another major uh goal for the center is to develop a community of Scholars focused on autopsy information metabolism and this is one of the reasons we developed the core centered around the types of government that we have which we'll describe and also again developing the infrastructure to support the community of Scholars focused on these studies and so we have um The Presence online if you'd like to follow us on Twitter go to our website LinkedIn um and engage with us there as well so the aim is organized with a RPI is with Reddick who introduced at the beginning as well as our associate director Dr Eric Posner um Advisory Board um as well as an internal Advisory Board and then we're comprised of a large number of um faculty at the University of New Mexico as well as again a team that's focused on today core which is what we the scientific core and um so again I'm the director we have an associate director as well as our technical director of who you'll meet and all of these activities really support the overall age of oh and um the center so as for the actual scientific um core leadership again I'm the director we have an associate director Dr Lou who's out of town could you join us um and Dr Larry splar who was actually really instrumental in the past few years um as a co-director but now has moved to help us develop a long-term um plan for the corps and then you'll meet um Dr sherina Desai who's our technical director who has a lot of expertise in all our specific equipment as well as um Dr Lee Chen who came on more recently as our associate technical director and they have all the scientific expertise for our equipment as well as the Top Gear Vision metabolism and then Dr Cassano is actually one of our mentored pis on the um AIDS Center but she's also assisting us with some of her special so I wanted to talk a little bit just to let um faculty and other users know about some opportunities um through the scientific core and so something that's new that's just come up and we've really tried to be um good about our communication with all the different users is that we do have now an annual access fee and that we ask for basically basic maintenance all of our equipment and access to all of the equipment that you'll hear about and this is a flat fee for annual thought fee of 400 for non-a members but that we do give a discounted rate for those who are eight members and um that we plan to gradually Institute hourly charges for the large equipment but that's not because and so for those who are interested in joining us again are one of our major roles to develop a community here and so we would love for anyone who's interested to become a membership and get membership in AIM and how to do this is that faculty pis join us by basically sending a paragraph about your research interests and how they align with autology information and or metabolism and then current CV and then give just a short research talk to the Ada community so that it really can engage the community as to how we might be able to collaborate to interact with each other and then that gives discounts um again to these support charges and so again we're really trying to build that um community of Scholars here that are focused on this autopsy information metabolism also we do have quite a few pilot awards that are available through the aim as it in general as well as the core and so the core specifically offers a voucher for metabolomic studies and Tool of five thousand dollars towards specific studies in metabolomics and this is done in collaboration with outside company that we have partnered with for many years and we facilitate this interaction so if you're interested in doing metabolomic studies please reach out to us and uh by voucher and we're happy to enable your students to be able to do these types of studies that are done with a company and then we also give facility use vouchers which are funds towards supplies to use for for equipment and so again that's usually available um annually and so if we look for emails from our Center then these are given out for people who are interested in using equipment in core and will help you um buy the supplies that are required to use equipment in the Forge towards your research as well as larger pilot projects in which we guide um basically can apply for a pilot project aligned with aim that are designed to help you get um Discovery data towards internal companies so again we have a lot of opportunities available if you have any questions feel free to email me or anyone reach out to the center and we're happy or about these opportunities I will turn this over to Sharina our technical director to talk about the specifics thank you Judy so we are located on the third floor of the Fitzgerald Falls or bits uh our main room is fits to be 84 but we also have recently added uh to MX rooms we know on me where you'll find me and I end up sitting a lot of times working on data analysis or helping others and also to 301a so I just I don't want to take away from any of the information that we have in store for the next few days but wanted to leave the overview our major and um smaller equipment so technically the the theme of our report facility is really centered around high content analysis so we have two machines currently by thermal Fisher called the salt Insight cx-7s in either a laser based excitation platform or an LED based transmission platform so the LED has an on-stage incubator to allow for time lapse microscopy and then we also have a robotic arm that's capable of handling up to two stacks of plates um and basically uh we have very similar but slightly different excitation capabilities it has seven different uh excitation um ranges and it can take up to a six Channel fluorescent image and then the highest mag that these machines can uh can utilize is 40x and so while you can get all the statistics from these machines and the software to complement it we also have a uh and they're the ZIP code microscope to help complement and get those higher resolution images that go with the statistics from the side effect so again not to delve too deeply but there are a number of Bio applications that range from many many different cellular processes where you can ask questions about cell cycle analysis or cell talks toxicity micronuclease assay Invasion assays co-localization General intensity spot basically this software can tell you the intensity size the area the location of all the pixels compared to all the pixels within the same channel and then compare them to all the pixels in other channels so potentially very powerful in being able to quantify your shape your protein uh there are protein intensities your mRNA intensities whatever you can think of and we're excited to show you all the different ways that these can be applied so those are primarily for samples that are adherent so adherent cells the nutrient being run magnetic small planes we can do slides but we also have an Imaging flow cytometer which can also do high content analysis or samples that are in suspension so because it is it's a closed cytometer in that it takes cells in suspension but it's really a microscope right so all of the data is coming from Imaging um images and so we have up to nine fluorescent channels two right field channels a side scatter channel so it is really the Perfect Blend of flow cytometry where you can get images in the thousands um but also get subcellular localizations you can ask questions about autophagy mitosis others basic cell biology questions microbiology and look at phagocytosis parasitology you can even look at some changes and even the interface between two cells so this is also you know just as powerful uh and we will be focusing on this for the next two days not today but this is our amnest cell stream Mark II um we also have metabolic analyzers uh in a 24 well and 96 well format so it's amazing these uh the top part of this sandwich here has a fiber optic fluorescent Center uh sensor that comes just above the cells and is able to be without a fluorescent tagging um basically the glycolysis or respiration of the cells and you can mix and match them with different uh drug deliveries that are delivered by pneumatic injection to be able to tell the profile the metabolic profile of the cells our newest major instrument which is the Highlight for today is the isoplexus um formally uh it's we actually have the isolite formally in the company isoplexus and we will be highlighting you know it's very um very complex capability for either complex or isoplex chip analysis and um I won't say anything more since we have a special guest speaker to delve deeply into what that means for your experiment um and it just briefly wanted to go over some of the smaller equipment um not as heavy with the analysis but still I think very helpful in supporting a lot of your work we have an Optima Max a tabletop Ultra centrifuge we have two candy dots which is our most highly visited machine uh capable of looking at Western blocks gels and more we have a basic guava flow cytometer it's just two lasers but with um six channels you can still read a lot of uh 404 combinations we have the nuclear factor which is really a transfection system designed to uh to optimize primary cells are very difficult to trans fat cells and we have a variety of cell plate leaders um that uh either our Standalone so they really help with your uh VCA assays your MTT assays uh this citation one also has basic microscopy function and we've coupled that with the c-force of metabolic analyzers in order to be able to count cells and therefore normalize their data we also have the Emax plus so we can replace all day long and what's really nice about these plate leaders is that they can leave bioluminescence fluorescence and absorbance we have dual injector to be able to automate your reagent delivery and you also have a shaping function and um and also temperature control so you can even grow leaves and do a yeast growth curve assay you can shape them in linear or older or the camera box can do very classic ecl substrate as well as fluorescent substrates so we have five major fluorescent channels which allows you to Overlay for example if you want to look at total protein versus phosphorylated protein and you can't resolve those sizes that's what makes the fluorescence a really powerful tool um we don't encourage putting animals in the machine but just to show that it is possible to look at something like gfp and we also of course do sting-free normalization which um isn't as widely used but it's a powerful technology where you can normalize not just to housekeeping genes which you know they're sometimes doubt as to whether or not your passing does not affect house conditions but you can normalize to the total protein loaded per well we also have the optimumide centrifuge so it's a tiny rotor but it can go up to 200k times achievement you can use the density gradient and of course they get things like exosomes and and really anything that you need for Ultra Central vacation but it's a fixed Android loader um so that's it with that I'll introduce Dr Lee Chen our newest addition to the Forum to just finish out our introduction good morning everyone I'm Dr Lee Chen I'm so proud of joining the important things uh January this year and I'm so happy to uh let you know we have experienced lots of growth especially in the uh five years here is a temple of August from last year yeah Justine the first first quarter of this year we had already Support over 40 bands and 80 customers and we have reach out over uh 1700 hours of usage to have this what would have been your growth and to better support our customers we also establish our steps for booking system uh you can book our time from this system either for the return we can talk about their experiments and uh like the the sample operation for the specific instruments and also for the new users who wants to use our foreign how to book the uh our our support and how to reserve the instruments and you can also uh put the one hour training for the share equipment for the three hours training for the future and decide that I learned for a alarm system we also established our SharePoint web page from this uh web page we can you can find all the instruments and the guide videos and like the instrument stacks and Sops other department things and the way other people were to support more customers on your research project thank you and that'll give it right thank you so we've found um an exciting few days and wanted to really take the time to allow uh users and interesting parties to take a deeper dive into some of our major instruments so today we are going to really focus on our newest instrument the isoplexus and with us we have Dr Daniels from the pitcherella lab to talk about their work with this platform we're also going to have later Lisa English and Keva um by to demonstrate both the uh both the instrument and the software they are held up they're applying in a lot of uh out of town so I have yet to hear but we do have uh Jamie here with us this morning tomorrow we're going to focus on the Agnes which is now part of scitec and so we're gonna go over some of our user data and then also have Dr Manuel novan to give the tech talk and um uh he's also going to have uh open consultations and look at users analysis so if you guys had any questions about how this machine could support your experiment not just in a classical close cytometry way but to kind of delve deeper into that high content analysis he's also going to be running a machine learning package to help users better analyze their data ISO plexus uh team will also be around for further consultation so I encourage anybody who's interested in this technology to come see them anytime in the afternoon tomorrow to talk about how they can building support your research on Thursday it's we're not going to have any major uh broadcasts but we are going to be on hand to take deeper dives into the data analysis of amnest next week we're going to focus on the seahorse data and we're going to be highlighting some of our major users and I'll also get a tech talk on Zoom from people on a company and then finally the last day you choose day the 27th we're going to be focusing on our bread and butter the CX-7 which we have lots of very experienced users and hopefully we'll be able to show you the real threats of different ways you can use this technology to answer many different types of questions autophagy information metabolism and more looking at various different applications so we're going to have their team on site and hopefully also learn a little bit about the Amira software platform that will be adding to the court facility so it's a standalone 3D uh 3D analytical software that works uh directly with the CX-7 software and also a basic overview of what our microscopy can do when we try to complement our cs7 so that's it most of all this will be held in the conference room when it comes to talks but we'll be floating around in the core facility as we highlight the machine thank you oh um uh sure we don't have it now but we will um we will find a way to share it with in the notes of as we make this available do you want to just copy and paste to phone because I think um presentation it's in the presentation but as it's part of uh so yeah we will get that on we will be short of put it in sometime in the future but um right now this is the school this is a screenshot but it's from the unmhsc dot sharepoint.com under their different sites more under UNM import users but um yes we'll definitely find a way to share that wait a minute thank you um yeah so I'm gonna stop right here and let um thank you so much you don't want me anywhere near me